| 貨號 | 規格 | 價格 |
| 4000-1 | 0.75ml | 1099 |
| 4000-2 | 1.5ml | 1999 |
產品介紹
EntransterTM-D4000是英格恩生物公司(Engreen Biosystem Co,Ltd.）最新研發合成的新型納米聚合物高效轉染試劑，該試劑采用納米技術合成，是新型的超越脂質體的低毒高效轉染試劑。
主要特點
脂質體升級版，難轉染細胞也能輕松應對，更好用！ 24孔板每孔只需要1ul，價格是脂質體轉染試劑的1/3.
用EntransterTM-D4000成功轉染的細胞
1、人源，遺傳變異細胞和正常組織來源細胞
AsPC-1，BNL CL2，HEK293，CFPEo，HUVEC，HLMEC，H-MVEC，MRC-5，HCS-2/8，HLF，D407，H9C2，SMC
2、人源，腫瘤細胞
A549，HuH-7，HepG2，HME4，A375，Hep-2，SW480，SKOV-3，IGROV1，K562，Jurkat，MCF7，MDA-MB-231，ZR-75-1，TCA8113，Eca-109，SGC-7901，HeLa，SiHa，Ca Ski，CaCo2，SAOS-2，PC-3，SHEP
3、其他動物來源細胞
B16BL6，B16F10，C2C12，C6，CHO，COS-7，CV-1，BHK-21，MDCK，PC12，BAEC，MLE-12，MLE-15，Neuro2A，PGHAM-1，RAW 264.7，EL-4，L929，LLC，3LL，NIH 3T3
各細胞系詳細轉染效率情況
| 細胞名稱 | 來源 | 轉染效率(%) |
| AsPC-1 | 人源，胰臟細胞 | 80% |
| BNL CL2 | 人源，胚胎腎細胞 | 70% |
| HEK 293 | 人源，胚胎腎細胞 | 90% |
| CFPEo | 人源，氣管上皮細胞 | 50% |
| HUVEC | 人源，臍帶靜脈內皮狀細胞 | 55% |
| HLMEC | 人源，肺血管內皮細胞 | 60% |
| H-MVEC | 人源，微血管內皮細胞 | 50% |
| MRC-5 | 人源，胚胎肺細胞 | 75% |
| HCS-2/8 | 人源，軟骨樣細胞 | 40% |
| HLF | 人源，肺成纖維細胞 | 65% |
| D 407 | 人源，視網膜色素上皮細胞 | 60% |
| A549 | 人源，肺癌細胞 | 90% |
| HuH-7 | 人源，肝癌細胞 | 63% |
| HepG2 | 人源，肝癌細胞 | 30% |
| HME4 | 人源，黑色素瘤細胞 | 90% |
| A375 | 人源，黑色素瘤細胞 | 70% |
| Hep-2 | 人源，喉癌細胞 | 70% |
| SAOS-2 | 人源，骨肉瘤細胞 | 50% |
| SKOV-3 | 人源，卵巢腺癌細胞 | 60% |
| IGROV1 | 人源，卵巢癌細胞 | 20% |
| K562 | 人源，慢性白血病細胞 | 30% |
| Jurkat | 人源，T淋巴細胞白血病細胞 | 45% |
| MCF7 | 人源，乳腺癌細胞 | 55% |
| MDA-MB-231 | 人源，乳腺癌細胞 | 70% |
| ZR-75-1 | 人源，乳腺癌細胞 | 70% |
| TCA8113 | 人源，舌鱗癌細胞 | 90% |
| Eca-109 | 人源，胃腺癌細胞 | 57% |
| SGC-7901 | 人源，胃腺癌細胞 | 48% |
| BGC-823 | 人源，胃腺癌細胞 | 60% |
| Panc-1 | 人源，胰腺癌細胞 | 40% |
| HeLa | 人源，宮頸癌細胞 | 80% |
| SiHa | 人源，宮頸癌細胞 | 70% |
| Ca Ski | 人源，宮頸表皮樣癌細胞 | 80% |
| CaCo2 | 人源，結腸癌上皮細胞 | 30% |
| SW480 | 人源，結腸腺癌細胞 | 80% |
| PC-3 | 人源，前列腺癌細胞 | 30% |
| SHEP | 人源，神經母細胞瘤細胞 | 70% |
| B16BL6 | 小鼠，黑色素瘤細胞 | 90% |
| B16F10 | 小鼠，黑色素瘤細胞 | 80% |
| C2C12 | 小鼠，胚胎肌原細胞 | 30% |
| C6 | 大鼠，腦膠質瘤細胞 | 60% |
| CHO | 中國倉鼠，卵巢細胞 | 90% |
| COS-7 | 猴SV40轉化腎細胞 | 80% |
| CV-1 | 非洲綠猴腎細胞 | 70% |
| BHK-21 | 倉鼠，腎細胞 | 80% |
| MDCK | 犬，腎上皮細胞 | 30% |
| PC12 | 大鼠，腎上腺嗜鉻細胞瘤細胞 | 33% |
| BAEC | 牛，動脈內皮細胞 | 50% |
| MLE-12 | 小鼠，肺上皮細胞 | 70% |
| MLE-15 | 小鼠，肺上皮細胞 | 70% |
| Neuro2A | 小鼠，成神經瘤細胞 | 70% |
| PGHAM-1 | 倉鼠，胰腺癌細胞 | 90% |
| RAW 264.7 | 小鼠，巨噬細胞 | 15% |
| EL-4 | 小鼠，胸腺瘤細胞 | 75% |
| L929 | 小鼠，成纖維肉瘤細胞 | 60% |
| LLC | 小鼠，路易斯肺癌細胞 | 75% |
| 3LL | 小鼠，路易斯肺癌細胞 | 65% |
| NIH 3T3 | 小鼠，胚胎纖維原細胞 | 40% |
| P19 | 小鼠，胚胎肉瘤細胞 | 60% |
低細胞毒性
由于EntransterTM-D4000非脂質體，無免疫原性，表現了極低的細胞毒性。
應用本產品文獻
5.Chinese Journal of Otology.2009(04):347-351.Study on transfection efficiency and cytotoxicity of 293T cells with different vectors containing Math1-EGFP gene.
6.FEBS Lett. 2010 Jul 2;584(13):2807-15. Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
7.Leuk Res. 2011 Feb 3. Specific killing of CCR9 high-expressing acute T lymphocytic leukemia cells by CCL25 fused with PE38 toxin.
8.J Biol Chem. 2011 Jul 27.Cep70 interacts with gamma-tubulin to localize at the centrosome and is critical for mitotic spindle assembly.
9.Cell Biochem Funct. 2011 Oct 26.The effects of myocyte enhancer factor 2A gene on the proliferation, migration and phenotype of vascular smooth muscle cells.
10.Parasit Vectors. 2011 Nov 9;4:213. DNA vaccination with a gene encoding Toxoplasma gondii GRA6 induces partial protection against toxoplasmosis in BALB/c mice.
11.J Cell Sci. 2012 May 28. Regulation of apoptosis by Bat3-enhanced YWK-II protein/APLP2 stability.
12.PLoS One. 2012;7(5):e37452. Exosome-related multi-pass transmembrane protein TSAP6 is a target of rhomboid protease RHBDD1-induced proteolysis.
13.J Appl Toxicol. 2012 Aug;32(8):635-41.Developing in vitro reporter gene assays to assess the hormone receptor activities of chemicals frequently detected in drinking water.
14.Respir Physiol Neurobiol. 2012 Oct 8. Effect of annexin A2 on hepatopulmonary syndrome rat serum-induced proliferation of pulmonary arterial smooth muscle cells.
